- 3 |
|
(Assessment of the impact of GMO of plant origin on rat progeny development in 3 generations)
.. , .. , .. , .. , .. , .. , ..
N.V. Tyshko, V.M. Zhminchenko, V.A. Pashorina, K.E. Selyaskin, V.P. Saprykin, N.T. Utembaeva, V.A. Tutelyan
,
Institute of Nutrition of Russian Academy of Medical Sciences, Moscow
- , . : 109240, , , . 2/14 : (495) 698-53-64
The publication presents the results of assessment of impact of genetically modified (GM) maize Liberty Link® on prenat al and post natal development of progeny of 3 generations oj Wist a r rats. A total of 630 adult animals and 2837pups were used in the experiment. The animals were divided into 5 groups which got. the diets with inclusion of maize: the animals of the experimental group got: the diet with the GM-maize, animals of the control group - with near isogenic conventional analogue of the GM-maize, animals of the 1, 2 and 3 reference groups - conventional varieties of maize «ROSS 144 MV», «ROSS 197MV», «Dokuchayevskaya 250 MV» respectively. The maize was included in the diet at maximum possible level not: isolating the balance of basic nutrients. Analysis oj the data obtained during the study did not reveal any impact of GM-maize on rat progeny development.Key words: reproductive function, genetically modified (GM) foods. GM-maize, the progeny development of rats
() ® - 3 . , 630 . 2837 . 5 , , : - , - 3 -« 144 MB», « 197 » « 250 » (1-, 2- 3- ). , . - - .
: , () , -,
: - |
Evaluation of corn grain with the genetically modified input trait DAS-59122-7 fed to pigs |
|
( DAS-59122-7 )
Stein HH, Rice DW, Smith BL, Hinds MA, Sauber TE, Pedersen C, Wulf DM, Peters DN.
Department of Animal and Range Sciences, South Dakota State University, Brookings 57007, USA. hstein@uiuc.edu
A growth performance experiment was conducted to assess the feeding value of a double-stacked transgenic corn grain for growing-finishing pigs. The genetically modified corn grain contained event DAS-59122-7, which expresses the Cry34/35Ab1 binary insecticidal protein for the control of corn rootworm. This modified transgenic grain is resistant to western corn rootworm and is also tolerant to herbicides containing the active ingredient glufosinate-ammonium. The modified grain (59122), a nontransgenic near-isoline grain (control corn), and a commercial corn (Pioneer brand hybrid 35P12) were grown in a 2005 production trial in individually isolated plots that were located 201 m apart. A total of 108 pigs were allotted to corn-soybean meal diets containing 1 of the 3 grains as the sole source of corn. There were 3 pigs per pen and 12 replicate pens per treatment. Pigs were fed grower diets from 37 to 60 kg, early finisher diets from 60 to 90 kg, and late finisher diets from 90 to 127 kg. Within each phase, data for ADG, ADFI, and G:F were calculated. At the conclusion of the experiment, pigs were slaughtered and data for carcass quality were collected. Differences between 59122 and the control corn were evaluated, with statistical significance at P<0.05. No differences in ADG, ADFI, or G:F between pigs fed the control corn and pigs fed the modified corn were observed during the grower, early finisher, or late finisher phases. For the entire experimental period, no difference between pigs fed the control and the 59122 corn were observed for final BW (128.9 vs. 127.1 kg), ADG (1.02 vs. 1.00 kg), ADFI (2.88 vs. 2.80 kg), or G:F (0.356 vs. 0.345 kg/kg). Likewise, no differences in dressing percentage (76.48 vs. 76.30%), LM area (49.8 vs. 50.4 cm(2)), 10th-rib back fat (2.20 vs. 2.12 cm), and carcass lean content (52.9 vs. 53.4%) were observed between pigs fed the control and the 59122 corn grain. It was concluded that the nutritional value of the modified transgenic corn grain containing event DAS-59122-7 was similar to that of the nontransgenic near-isoline control.
. DAS-59122-7, Cry34/35Ab1. , . (59122), () ( 35P12 "Pioneer") , 201 . , , , 108 . "grower" 37 60 , "early finisher" 60 90 , 90 127 "late finisher". : ADG, ADFI G: F. , . 59122 P <0,05. ADG, ADFI G: F , , , . , , 59122 , BW (128,9 127,1 ), ADG (1,02 1,00 ), ADFI (2,88 2,80 ) G : F (0,356 0,345 / ). , DAS-59122-7 .
PMID: 19098236
: Evaluation of corn grain with the genetically modified input trait DAS-59122-7 fed to growing-finishing pigs.
: - |
A three generation study with GM Bt corn in rats |
|
( : )
Department of Biology, Faculty of Science, Hacettepe University, Beytepe Campus, Ankara, Turkiye. aykilic@hacettepe.edu.tr
For the last ten years, in accordance with the increased use of genetically modified (GM) foods for human and livestock, a large number of feeding studies have been carried out. However, the evidence is still far from proving whether the long-term consumption of GM foods poses a possible danger for human or animal health. Therefore, this study was designed to evaluate the effects of transgenic corn on the rats that were fed through three generations with either GM corn or its conventional counterpart. Tissue samples of stomach, duodenum, liver and kidney were obtained for histopathological examinations. The average diameter of glomeruli, thickness of renal cortex and glomerular volume were calculated and number of affected animals/number of examined animals for liver and kidney histopathology were determined. Amounts of urea, urea nitrogen, creatinine, uric acid, total protein, albumin and globulin were determined; enzyme activities of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma glutamyltransferase, creatine kinase and amylase were measured in serum samples. No statistically significant differences were found in relative organ weights of rats within groups but there were some minimal histopathological changes in liver and kidney. Changes in creatinine, total protein and globulin levels were also determined in biochemical analysis.
, - . , , . , , , , , , , ; , , , , . : ( ).
PMID: 18191319
: A three generation study with genetically modified Bt corn in rats: Biochemical and histopathological investigation.
: - bt- |
Evaluation of Bt corn on mouse testicular development by flow cytometry |
|
( Bt- )
Department of Chemistry and Biochemistry, South Dakota State University, Brookings, South Dakota 57007, USA.
The health safety of Bt (Bacillus thuringiensis) corn (Zea mays L.) was studied using mouse testes as a sensitive biomonitor of potential toxic effects. Pregnant mice were fed a Bt corn or a nontransgenic (conventional) diet during gestation and lactation. After they were weaned, young male mice were maintained on the respective diets. At 8, 16, 26, 32, 63, and 87 days after birth, three male mice and an adult reference mouse were killed, the testes were surgically removed, and the percentage of germ cell populations was measured by flow cytometry. Multigenerational studies were conducted in the same manner. There were no apparent differences in percentages of testicular cell populations (haploid, diploid, and tetraploid) between the mice fed the Bt corn diet and those fed the conventional diet. Because of the high rate of cell proliferation and extensive differentiation that makes testicular germ cells highly susceptible to some toxic agents, it was concluded that the Bt corn diet had no measurable or observable effect on fetal, postnatal, pubertal, or adult testicular development. If data from this study were extrapolated to humans, Bt corn is not harmful to human reproductive development.
Bt (Bacillus thuringiensis) (Zea mays L.) . . , (8, 16, 26, 32, 63 87 ) , , (, ) . - , , , Bt- , . , Bt- .
PMID: 15053558
: Evaluation of Bt (Bacillus thuringiensis) corn on mouse testicular development by dual parameter flow cytometry.
: - bt- |
Long term feeding of Bt-corn--a ten-generation study with quails |
|
( Bt- )
Institute of Animal Nutrition, Federal Agricultural Research Centre, Braunschweig, Germany. gerhard.flachowsky@fal.de
A ten-generation experiment with growing and laying quails were carried out to test diets with 40 (starter) or 50% (grower, layer) isogenic or transgenic (Bt 176) corn. Feeding of diets containing genetically-modified corn did not significantly influence health and performance of quails nor did it affect DNA-transfer and quality of meat and eggs of quails compared with the isogenic counterpart.
, 40 () 50% (Bt 176) . : , , -, .
: - bt- |
Nutritional evaluation of genetically modified maize corn performed on rats |
|
( )
Institute of Animal Nutrition, Research Institute of Animal Production, Hlohovská 2, 949 92 Nitra, Slovak Republic. chrenko@vuzv.sk
The aim of this study was to determine the composition and nutritional value of conventional and transgenic, so-called Roundup Ready (RR) maize with an introduced gene of glyphosate resistance. Crude protein, crude fibre, ash, fat, starch, sugar, amino acids, fatty acid and macroelement levels were determined by chemical analysis. In both maize lines a low level of Ca (0.15 g.kg-1 DM) and of the essential amino acids lysine and tryptophan (2.6 and 1.7 g.kg-1 DM, respectively) were observed. In the biological experiment carried out on rats the tested maize lines were the only dietary sources of nitrogen, thus, the experimental diets contained 9% CP in dietary dry matter. In the feeding experiment no significant differences in the protein efficiency ratio (PER) were observed between groups receiving conventional or transgenic maize (1.51 and 1.41, respectively). Also almost equal results were obtained in the balance experiments. Both maize lines revealed a high nitrogen digestibility (84.9 and 84.5%, respectively) and the net protein utilization amounted to 63.5 and 63.2%, respectively. From these results can be concluded that regarding nutrient composition and utilisation, genetically modified (RR) maize is equivalent to isogenic maize.
Roundup Ready (RR) , . , , , , , , . Ca (0.15 g.kg-1 DM) (2.6 1.7 g.kg-1 DM). , , , , 9% CP. (PER) , (1,51 1,41, ). (PER) , (1.51 1.41). . (84,9 84,5%), 63,5 63,2% . , (RR) .
PMID: 12391907
: Nutritional evaluation of genetically modified maize corn performed on rats.
: - |
| : | [1] |
